Guava suite software provides an intuitive user interface and offers acquisition. The millipore guava easycyte ht flow cytometer is a flexible, compact and reliable high throughput benchtop system. The focus of this buyers guide is to discuss some of the key components and features of the instruments and to highlight some tips and considerations for selecting a flow cytometer system for your. A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. As part of our complete flow cytometry solution, or service agreements are.
However, many flow cytometers are expensive to purchase andor use, and many do not offer effective automated handling of samples, so that extensive operator time is. The customizable laser and optical configurations of novocyte offer a high degree of flexibility while providing complex cell analysis capabilities. The instruments compact design is suited for basic research as well as advanced immune monitoring. Used flow cytometry cell counting sorting biosurplus. The manual loader holds two separate tubes, allowing the user to progress. Setup procedure for guava easycyte with a single laser important note. The culmination of over a decade of flow cytometry expertise, these instruments use smaller sample, generate less waste, and are easier to use and maintain than traditional flow cytometers all while providing. The guava easycyte ht system users guide provides detailed. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size.
Emd millipore has thoroughly tested the operation of guavasoft software on windows xp, but. Additional recommendations when collecting and analyzing your samples. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays. I have a demonstration in a couple of days and im interested in researchers experiences. This flow cytometer system produces dependable data for a wide range of multicolor cellular and bead assays in a highly user friendly fashion and can handle 96well plates and up to 10 tubes in a single load.
The guava easycyte ht doesnt require sheathing fluid, so you can. Thermo fisher scientific certifies that the attune nxt flow cytometer conforms to relevant directives to bear the ce mark. This translates into less complexity, smaller samples, and minimal waste, saving you both time and money. Do not open the instrument or attempt to perform any internal maintenance. This combination of detection methods results in the most accurate method or way to count cells. It also enables other very powerful cell analysis and qc. Emd millipore has thoroughly tested the operation of guavasoft software on microsoft windows xp and. The instrument also conforms to the ul and cancsa general requirements 61010. Navios is equipped with electronics that provide accurate and efficient digital signal processing at high event rates over a wide dynamic range of fluorescence intensities. Emd millipore has thoroughly tested the operation of guavasoft software on. For more detailed instructions on system operation details, please consult the millipore guava operation manual. Guava easycyte instrument features easycyte flow cytometers use novel microcapillary technology that enables a compact userfriendly format, which is powerful enough to run your most complex assays.
Oct 07, 2016 millipore guava easycyte flow cytometer is available from the lab world group. Guava incyte software includes features that can be applied to control user access to data files and methods, which is useful in multi user environments. Attune acoustic focusing cytometer user guide 7 safety information or instrument safety information. Orflo uniquely integrates flow cytometry fluoursence detection with the coulter counter principle. Ion sphere particles quality assessment for the ion proton and ion s5 systems using the guava easycyte 5 benchtop flow cytometer publication number man0007496 revision b. A guava easycyte ht 2laser flow cytometer millipore was used with excitation by the blue 488nm laser and the gain controls at their default settings yellow and green fluorescence or set to 9. The attune nxt flow cytometer is a class i laser product per center for devices and radiological health cdrh regulations and eniec 60825. Flow cytometers, reagents, software beckman coulter. All guava instruments use microsyringebased fluidics, which eliminates the need for sheath fluid and enables the user to obtain accurate cell counts in the samples. Setup procedure for guava easycyte with a single laser. The instrument accommodates 96well plates and up to 10 tubes in a single load. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less. Flow cytometers, cell sorters and cell preparation systems. Biosurplus has these instruments used for flow cytometry for sale with a wide range of features, a few of which are discussed here.
Flow cytometers life science classifieds new and used. Novocyte is a high performance benchtop flow cytometer designed for all levels of users and all types of laboratories. Guava incyte brings a new level of analytical power to flow cytometry. We are considering either accuri c6 or guava flow cytometers and we would greatly appreciate if we get a good feedback from those who used these machines for issues like running costs, maintanance and drawbacks of each. The first place to look for new and used flow cytometers. Spectrometers shared equipment authority rice university. Guava easycyte flow cytometers from luminex corporation. Plus, the flow cell is selfaligning and user replaceable to reduce downtime and service visits. The 2019 invitrogen attune nxt flow cytometer offers exceptional performance in a user friendly benchtop instrument with up to 4 lasers and 16 parameters. Rapid, effective and userfriendly immunophenotyping of. To avoid exposure to laser radiation or electric shock, follow these guidelines. In addition, it is equipped with a highthroughput sampler able to automatically measure samples directly from a 96well plate or via micro. Find a flow cytometer for sale and auction at labx. Optimize your flow cytometry best practices for sample preparation, staining and analysis duration.
All these features make guava easycyte mini a fully functional and very attractive flow cytometer. Cytometry accuri c6 vs guava cytometersfeedback needed. Guava easycyte ht bg system is a flow cytometer for performing flow cytometry experiments. The guava viacount, guava check, and guava easycheck software modules allow samples to be run only at the medium flow rate. Guava easycyte system users guide cytotoxicity apoptosis. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements. The results we obtained are highly reproducible with superb intraexperiment variation coefficients. Setup procedure for guava easycyte with two lasers important.
Manual adjustments can also be made after data load by following these instructions. This is the next generation leveraging the laser and optics technology of our successful cytoflex research platform. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers all. Our group has used the guava extensively for well over a year. For best performance, emd millipore corporation recommends that you use the medium flow rate, except for the guava cell cycle software module, which uses low or very low flow rates only. Navios flow cytometer the navios offers up to three high powered solid state, independentlyfocused diode lasers with an innovative integrated optics flow cell design. Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. The flowsight and imagestream imaging flow cytometers add quantitative, microscopy imaging of every cell to your flow cytometry. Flow cytometer guava easycyte available for sale at the lab world group. Figure 11 bd facscalibur system with bd facs loader option. Some of our popular manufacturers include beckmancoulter, bd biosciences bectondickinson and guava. Rapid, precise, and accurate counts of symbiodinium cells using the guava flow cytometer, and a. The navios flow cytometer offers a solution for advanced cytometry applications with workflows for high. We present the utter option of this ebook in pdf, txt, djvu, epub, doc forms.
We also enjoyed its portability, since we were able to share it with other research groups and use it directly in the cell culture. The guava easycyte flow cytometry systems are easy to use and deliver complete and comprehensive cell analysisright on your benchtop. Guava easycyte ht bg flow cytometer shared equipment. This budgetfriendly instrument is capable of detecting up to 17 parameters with enhanced sensitivity and resolution.
Attune nxt acoustic focusing cytometer the invitrogen attune nxt acoustic focusing cytometer is a benchtop cytometer that uses acoustic pressure to confine the injected particles to a tight central line as the sample passes through the optical cell for interrogation. May 27, 2016 scientists have many instrumentation and reagent options when selecting andor purchasing a flow cytometer system to address their research needs. Guava easycyte mini from guava technologies biocompare. Amnis cellstream amnis flowsight amnis imagestream x mk ii guava easycyte guava muse. Has anyone experience with the easycyte 5 flow cytometer from millipore. Millipore guava easycyte ht flow cytometer system available from the lab world group. Millipore guava easycyte ht flow cytometer system youtube. Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology. Because the scale of flow data is relative, this is acceptable. An optional, auto mated sample loader is also available. You can change these default preference sets in the cytometer specific preferences. Ion sphere particles quality assessment for the ion proton. The guava muse flow cytometer is easy to use, fast and reliable, far less expensive than other bench top sytems.
Use the following metrics together to evaluate a sample, but not as. Has anyone experience with millipores guava flow cytometer. The navios ex flow cytometer offers a solution for advanced cytometry applications with workflows for high throughput laboratories. Millipore guava easycyte flow cytometer is available from the lab world group. Sorting has the advantage of isolating particular cells for further study away from the nonlabeled of cells. The aim of this study was to adapt the immunohistochemical. When you choose a beckman coulter solution you receive the a high. The university of sydneys major institute for medical research seeks to answer questions about the human body in health and in illness. These simple fluidics enable a selfaligning flow cell that can be removed for cleaning by the user. The guava easycyte 5 flow cytometer offers absolute cell counts and five parameters 3 fluorescent colors, 2 light scatter, from an industry standard blue laser. Determinations of algal numbers using the guava flow cytometer.
Ion sphere particles quality assessment user bulletin 7. Millipore, muse, and guava are registered trademarks or trademarks of. This instrument is in great condition and has been verified by our company technical staff to. If you created a worklist already, click start worklist. Turn off the power to the system before removing the flow cell. Millipore guava ht with plate handler protocol for multiplex. The guava instruments are based on microcapillary flow. Find msds or sds, a coa, data sheets and more information.
Setup procedure for guava easycyte with two lasers. The macsquant analyzer 16 the macsquant analyzer 16 is engineered to expand the revolutionary automation of the macsquant analyzer line of flow cytometers. Used guava technologies pca flow cytometer for sale. Plus, since the flow cell is selfaligning and user replaceable. Flow cytometry cambridge scientific used lab equipment. The guava easycyte flow cytometry systems are easy to use and deliver. Assay results with statistics automatically calculated and. The guava easycyte plus guava technologies, hayward, ca, us is the first, personal, benchtop flow cytometer designed to address these limitations. Microcapillary flow cytometry at the heart of every guava system is a patented, microcapillary flow cell that eliminates the need for sheath fluid. It facilitates fully automated data acquisition in a 24tube or 96well plate format. Its single sample loader offers reliable tube sampling. Safety on page 68 for the complete the chemical safety alert words four safety alert words appear in life technolo gies user documentation at points in the document where you need to be aware of relevant hazards. Rapid, precise, and accurate counts of symbiodinium cells. This technology offers some advantages for general flow cytometry but the instruments are different from traditional flow cytometry and the performance of legendplex is also different.
Guava easycyte flexible, intuitive, and affordable cytometry for all your needs watch an overview video on the guava easycyte systems our microcapillary flow cytometry systems are simpler to operate than traditional sheathfluid based instruments and are far easier to maintain. The guava easycyte flow cytometers use up to three lasers 488, 642, and 405 nm, to achieve. The guava easycyte flow cytometry systems are uncomplicated instruments that deliver complex cell analysis right on your benchtop. Phd graduate student kyoto prefectural university of medicine kyoto, japan. Multiply the m1 cellsml by the original sample volume to calculate the total number of recovered isps. We offer an assortment of used and refurbished flow cytometers and cell sorters. May 31, 2017 millipore guava easycyte ht flow cytometer system available from the lab world group. We offer specialized instruments for routine or high complexity flow cytometric analysis designed with a deep understanding of the clinical workflow and the needs of the hematopathologist. The cytoflex flow cytometer, the first introduction to the cytoflex platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. With amnis chargecoupled device, timedelayed integration ccdtdi technology, the cellstream provides unparalleled high sensitivity in a highly customizable flow cytometer. Shared equipment authority sea we provide sophisticated research equipment and facilities to all members of the rice university community, as well as support and open instrumentation usage to outside companies, nanobioenviro startups and other universities upon availability. Comparison and evaluation of seven different bench. Blue 488 nm 150 mw and green 532 nm 100mw with 8 fluorescent detection channels, forward and side scatter.
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